Isolation Of Bacteria

هنتكلم عن فصل البكتيريا فى المعامل
موضوع شيق وجميل وياريت تستفيدوا منه
مع تحياتى
المدير \Dr.ahmed
اليكم الموضوع


1. Loop From Pathogen (+ve) Petri Dish
2. Strike on N.A. Petri Dish As Dil.
3. Incubate for 24hrs.
4. Purification Step:
a. Strike pathogen on its specific medium
E.Coli MacConkey Agar or E.M.B.
Staphylococcus Mannitol Salt or BAIRD-PARKER Agar
Pseudomonas Pseudo. Selec. Agar
5. Incubate 24hrs.
6. Two Decisions
a. Put in the fridge
b. Prepare Bac. Suspension for preparatory tests

Prparatory Test

1. Preparing Bacterial Suspension:
a. Prepare 3 Slope Tubes
b. Strike 3 Path. On Three Slope Tubes
c. Incubate for 24hrs.
d. Prepare Saline Soln. (About 2%)
e. Put 9ml. Saline in the first tube
f. Write Zero on this tube
g. Pour Saline Soln. in the first slope tube
h. Keep Saline tube Sterile
i. Skake Well to make Bac. Suspension You Can Use sterile loop to mix it well
j. Pour saline soln. in the tube again
k. Take 1ml. into 9ml. Saline Soln. (make serial Dil. Into 10-8 )
Note:
Shake Very Well during Dil.
l. Choose Tubes no. (6,7,
m. Take 1ml. from each tube into Petri dish
n. Pour N.A. Medium on it
o. Incubate for 24hrs.
Note:
E.Coli & Pseud. At 10-6
Staph. At 10-5
2. Preparatory Test:
a. Take three tubes (2 N.B. + Tween80 2%) & (1 L.B. Tween80 2%)
b. Put them in four y rows each one contain three tubes
c. Put E.Coli. & Staph. 10-6 (1ml.)
d. Put Pseud. 10-5 (1ml.)
e. Incubate 24hrs.
f. Strike Tubes on three Petri dishes
E.Coli MacConkey Agar or E.M.B.
Staphylococcus Mannitol Salt or BAIRD-PARKER Agar
Pseudomonas Pseudo. Selec. Agar

The Lowest Conc. For the three Pathogens (Same Dil.) Is the suitable Conc.